FAQs - T&L Biotechnology

Nqe Lus Nug ◢

Q.
1. Dab tsi yog qhov subtype ntawm CD3 antibodies?

A.
CD3E
Q.
2. Puas yog nws zoo dua rau cov tshuaj tiv thaiv CD3 thiab CD28 soluble rau ntawm lub phaj los yog ua kom lawv nyob rau hauv cov kab lis kev cai nruab nrab tov thaum siv los cog cov tib neeg T hlwb?

A.
Ob txoj kev yog ua tau
Q.
3. Ntev npaum li cas lub raj mis kab lis kev cai coated nrog NK reagent kit 2.0-A yam yuav khaws cia?

A.
Yuav tsis muaj teeb meem hauv ib lub lis piam.
Q.
4. PBMCs puas tuaj yeem rov muaj dua tom qab cryopreservation siv los cog NK hlwb?

A.
PBMCs uas tau khaws cia thiab rov zoo tuaj yeem siv rau kab lis kev cai NK hlwb, tab sis kab lis kev cai cuam tshuam tsis zoo li PBMCs tshiab.
Q.
5. MSC additives tuaj yeem khaws cia ntawm 4 ℃ tom qab qhib?

A.
Xya hnub.

Q.
6. Dab tsi tsis yog siv rau kev sib cais ntawm tib neeg Vitronectin protein (cov khoom naj npawb GMP-TL651)? Puas muaj kev xav tau rau calcium thiab magnesium ions?

A.
DPBS lossis cov kab lis kev cai tsis muaj ntshav qab zib tuaj yeem siv tau. Nws tsis pom zoo kom muaj calcium thiab magnesium ions nyob rau hauv qhov tsis zoo.
Q.
7. Yuav ua li cas hloov ED50 rau chav tsev?

A.
Unit/mg = 106/ED50 (ng/ml)
Q.
8. Dab tsi yog lub xov tooj ntawm tes purity thiab kev sib cais ua haujlwm tom qab sib nqus hlaws sib cais?

A.
Purity yog txhais raws li qhov kev faib ua feem ntawm lub hom phiaj sau cov hlwb thiab tuaj yeem txheeb xyuas los ntawm kev ntws cytometry. Kev sib cais ua kom zoo yog txhais raws li qhov kev faib ua feem ntawm cov hom phiaj rov qab txheeb ze rau lub hom phiaj hauv cov qauv qub.
Q.
9. Dab tsi yog qhov purity ntawm T hlwb tom qab siv GMP-TL603 cais & ua kom sib nqus hlaws?

A.
Lub purity sib txawv nyob ntawm seb lub cell viability/status/T cell ratio ntawm tus qauv.
Q.
10. Thaum twg peb yuav tsum ua qhov kev sim kis tus kab mob tom qab siv GMP-TL603 cais & ua kom cov hlau nplaum sib nqus thaum lub sij hawm CAR-T sim? Peb puas yuav tsum tau tshem cov hlaws sib nqus thaum lub sij hawm kis tus kab mob?

A.
Cov neeg siv khoom sim kis tus kab mob ntawm 24h, 48h lossis 72h, nrog 48h yog qhov ntau tshaj. Thaum tus kab mob kis tau, cov hlaws hlau nplaum tuaj yeem khaws cia.

Q.
11. Puas yog cov hlaws sib nqus tseem ua haujlwm thaum muab tso rau ntawm -20 ℃?

A.
Tsis pom zoo rau rov siv dua.
Q.
12. Puas tuaj yeem GMP-TL603 cov kab ntawv sau ncaj qha los ntawm kev ntws cytometry?

A.
Ua tsis tau, Nws qhia kom tshem cov hlaws sib nqus ua ntej ua ntej kuaj cov hlwb
Q.
13. Puas yog GMP-TL603 magnetic hlaws yuav tsum tau muab tshem tawm thaum sau cov hlwb tom qab ua kom tiav thiab nthuav tawm?

A.
Sib nqus hlaws yuav tsum tau muab tshem tawm, cov hlaws sib nqus dav dav thiab cov kab hlau nplaum tuaj yeem ua tau raws li daim ntawv thov.
Q.
14. Dab tsi yog qhov particle loj ntawm NanoSep™ CD3/4/8 seperation magnetic hlaws (TL-622, TL-623, TL-624)? Cov hlaws sib nqus puas xav tau cov kab sib cais? Puas yog magnetic hlaws biodegradable?

A.
50nm ua. Sib nqus hlaws yuav tsum tau mus los ntawm cov kab sib cais (Nws yog sib xws nrog Miltenyi MACS txhua), uas yog biodegradable.
Q.
15. Puas muaj cov hlaws sib nqus ua pov thawj kev nyab xeeb?

A.
Cov hlaws liab qab ntawm micrometer magnetic hlaws (GMP-TL603) thiab nano magnetic hlaws (TL-622, TL-623, TL-624) tau ua tiav kev soj ntsuam kev nyab xeeb thiab pov thawj ntawm cov tsiaj qib.