Recombinant Tib Neeg PDGF-BB Protein

Tshuag: Hauv Tshuag
Qauv: GMP-TL644

Tiv tauj peb

Kev piav qhia

Cov ntaub ntawv

Cim xeeb

Cov ntaub ntawv

PDGFs yog disulfide-linked dimers uas muaj ob 12.0-13.5 kDa polypeptide chains, xaiv PDGF-A thiab PDGF-B chains. Peb qhov tshwm sim PDGFs, PDGF-AA, PDGF-BB thiab PDGF-AB, yog cov muaj zog mitogens rau ntau hom cell, nrog rau cov leeg nqaij, cov nqaij mos sib txuas, cov pob txha thiab pob txha mos, thiab qee cov qe ntshav. Cov PDGFs khaws cia hauv platelet α-granules, thiab raug tso tawm thaum ua kom platelet. PDGFs tau koom nrog ntau cov txheej txheem lom neeg, suav nrog hyperplasia, chemotaxis, embryonic neuron kev loj hlob, thiab kev ua pa tubule epithelial cell. Ob qhov sib txawv signaling receptors ntawm PDGFs tau txheeb xyuas thiab npe PDGFR-α thiab PDGFR-β. PDGFR-α yog qhov muaj txiaj ntsig zoo rau txhua tus ntawm peb daim ntawv PDGF. Ntawm qhov tod tes, PDGFR-β cuam tshuam nrog PDGF-BB thiab PDGF-AB nkaus xwb.

Cytokines
Expression Host	HEK293 hlwb
Synonym	PDGFBB(Platelet-Derived Growth Factor-BB), PDGFB, FLJ12858, PDGF2, SIS, SSV, c-sis Glioma-derived growth factor(GDGF), Osteosarcoma-derived Growth Factor(ODGF)
Protein Sequence	Lub DNA ib ntus encoding tib neeg PDGF-BB (GenBank: CAA45383.1) tau qhia nrog polyhistidine lossis ntawm C-terminus.
Molecular Mass	Lub Recombinant Tib Neeg PDGF-BB muaj 115 amino acids thiab kwv yees ib molecular loj ntawm 13.1 kDa.
QC Test Purity	> 90% raws li kev txiav txim los ntawm SDS-PAGE.
Endotoxin
Kev ua si	Kev txiav txim siab los ntawm koob tshuaj-nyob ntawm kev txhawb nqa ntawm kev loj hlob ntawm Balb / c 3T3 hlwb. Qhov kev cia siab ED₅₀ rau cov nyhuv no yog ≤100ng / mL.
Kev tsim qauv	Lyophilized los ntawm sterile PBS, pH 7.4. Feem ntau 6% mannitol yog ntxiv los tiv thaiv ua ntej lyophilization.
Kev ruaj ntseg	Kev npaj lyophilized tuaj yeem khaws cia ntawm -20 ℃. 6 lub hlis ntawm -20 ℃ nyob rau hauv sterile tej yam kev mob tom qab reconstitution. 12 lub hlis ntawm -80 ℃ nyob rau hauv sterile tej yam kev mob tom qab reconstitution.
Cia	Pom zoo kom aliquot cov protein rau hauv cov khoom me me tom qab rov tsim dua nrog dej rau kev txhaj tshuaj, ib txwm saline lossis PBS, thiab khaws cov diluted concentration siab dua 100μg / mL. Tsis txhob rov qab khov-thaw cycles.