0102030405
GMP-TL101 Kuzindikira kwa Anti-Human CD3 mAb Antigen, kutulutsa ma siginecha ndi kuyambitsa kwa T cell
Zowonetsa Zamalonda
Anti-Human CD3 mAb (GMP TL101) idapangidwa kuti ithandizire kuzindikira kwa antigen, kutulutsa ma siginecha, ndi kuyambitsa kwa T cell. Antibody monoclonal iyi ndi gawo lofunikira kwambiri pazofufuza komanso zamankhwala zomwe zimakhudzana ndi kusintha kwa T cell.
Zofunika Kwambiri
● Kusintha kwa Chizindikiro: Kumagwirizanitsa mogwira mtima kuzindikira kwa antigen ndi kutulutsa zizindikiro, zofunika kwambiri kuti ma T cell ayambe.
● Kupanga kwa GMP: Kupangidwa pansi pamikhalidwe yovuta kwambiri ya GMP kuti zitsimikizire mtundu wa malonda ndi kuchulukitsidwa.
● Kukhazikika Kwapamwamba: Kukhazikika kwapamwamba kwa CD3, kuwonetsetsa kulunjika kolondola komanso zotsatira zochepa zomwe zimachokera.
● Kugwiritsa Ntchito Kwakukulu: Ndikoyenera kugwiritsidwa ntchito zosiyanasiyana, kuphatikizapo flow cytometry, cell culture, ndi kafukufuku wochiritsira.
Mapulogalamu
● Kafukufuku wa Immunological: Chofunika kwambiri pophunzira ma siginecha a T cell receptor (TCR) ndi kuyambitsa njira.
● Kukula kwa Mankhwala: Zothandizira pakupanga mankhwala opangidwa ndi T cell, kuphatikizapo CAR-T therapy.
● Maphunziro a Zachipatala: Oyenera kutsata ndondomeko zofufuza zachipatala zomwe zimafuna ma antibodies apamwamba, ogwirizana ndi GMP.
Tsatanetsatane ndi Kuyika
● Zofotokozera:
Imapezeka mumitundu ingapo kuti igwirizane ndi zosowa zosiyanasiyana zoyesera: 100μg, 500μg, ndi 1mg.
Miyezo yapamwamba imatsimikizira kusasinthasintha ndi kudalirika.
● Kuyika:
Amaperekedwa ngati wosabala madzi kapena lyophilized ufa mosavuta ntchito ndi yabwino kusunga.
Zoyikidwa kuti zisunge umphumphu ndi zogwira mtima panthawi yoyendetsa ndi kunyamula.
Kusungirako ndi Kukhazikika
Kusungirako
Kuti mugwiritse ntchito kwakanthawi kochepa, sungani pa 2-8 ° C.
Kuti musunge nthawi yayitali, sungani pa -20 ° C kuti musunge bata ndi zochita za antibody.
Pewani kuzizira kobwerezabwereza kuti mupewe kuwonongeka.
Kukhazikika
Imasunga bata pansi pamikhalidwe yovomerezeka yosungira, kuonetsetsa kuti ntchito yodalirika ikugwira ntchito pa kafukufuku.
Zapangidwira kuti zikhale zolimba, zomwe zimatsimikizira kugwira ntchito kwa nthawi yaitali.
Malangizo Ogwiritsa Ntchito
Sungunulani antibody mu nkhokwe yoyenera kapena sing'anga musanagwiritse ntchito.
Konzani ndikugwiritsa ntchito yankho molingana ndi zoyeserera kuti mupeze zotsatira zolondola komanso zobwerezedwanso.
Tsatirani ndondomeko zoyenera pakugwiritsa ntchito kwanu, kaya ndi flow cytometry, immunohistochemistry, kapena magwiridwe antchito.
Chenjezo
• Izi zimangogwiritsidwa ntchito pa kafukufuku ndipo sizoyenera kuwunika zachipatala kapena kuchiza.
• Gwirani chitetezo molingana ndi malangizo omwe aperekedwa kuti asunge ntchito yake ndi kukhulupirika.
• Pazovuta zilizonse kapena mafunso, chonde lemberani gulu lathu laukadaulo kuti muthandizidwe.
• Konzani kafukufuku wanu ndi ma antibodies athu osinthika komanso odalirika. Kuti mudziwe zambiri zamalonda ndi chithandizo, pitani patsamba lathu.
| Ma antibodies a Monoclonal | |
| Mapulogalamu | Njira yosungunulira: kuchuluka kwa 500 ng/mL kumalimbikitsidwa, pomwe ma T cell amatha kutsegulidwa ndipo ma CD3⁺CD56⁺T amatha kukulitsidwa kwambiri. |
| Zochita zamoyo | Mlingo womangiriza ndi ma cell a Jurkat suyenera kuchepera 90% |
| Njira yokutira: | 10 mL PBS buffer (5μg/mL anti-anthu CD3) imayikidwa mu botolo la T75 pa 4°C usiku wonse. Chonde chotsani PBS ndikutsuka botololo ndi saline mofatsa katatu musanagwiritse ntchito. |
| Endotoxin | |
| Expression Host | CHO maselo |
| Kupanga | Amaperekedwa ngati njira yosankhira 0.22μm mu PBS, PH 7.4 |
| Kuyeretsa: | Mapuloteni A oyeretsedwa kuchokera ku cell culture supernatant |
| Kuyesedwa kwa QC Kuyera | > 95 % malinga ndi SDS-PAGE |
| Kukhazikika & Kusunga | Miyezi 24 pa 2 ℃ mpaka 8 ℃. Pewani kuzizira kobwerezabwereza |
| Chala chachikulu | Zambiri zamafayilo |
 | Chithunzi cha GMP-TL101_SDS |
| GMP-TL101_Product Sheet.pdf |