Recombinant Human bFGF mapuloteni

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Basic fibroblast growth factor (bFGF), yomwe imadziwikanso kuti FGF2, ndi membala wa banja la fibroblast growth factor (FGF). Ndi chinthu chodziwika bwino cha chemotactic ndi mitogenic cha mitundu yambiri ya maselo, ikuwoneka kuti ikukhudzidwa ndi kukonzanso minofu yowonongeka, monga kuchiritsa zilonda, kukonzanso mitsempha, kuvulala kwa ubongo (TBI). Mapuloteni a m'banja ili amatenga gawo lalikulu pa nthawi ya chitukuko chaubwana, kukula pambuyo pa kubadwa ndi kusinthika kwa mitundu yosiyanasiyana ya minyewa, polimbikitsa kuchulukana kwa ma cell ndi kusiyanitsa. FGF-basic ndi yopanda glycosylated, heparin-binding kukula factor yomwe imawonetsedwa mu ubongo, pituitary, impso, retina, fupa, testis, adrenal gland, chiwindi, monocytes, epithelial cell ndi endothelial cell. bFGF ndi gawo lofunikira kwambiri la chikhalidwe cha embryonic stem cell. Kuphatikiza apo, puloteni ya bFGF ndi mapuloteni a heparin omwe amamanga cationic omwe amakhudzidwa ndi matenda osiyanasiyana kuphatikizapo angiogenesis ndi kukula kwa chotupa cholimba. Chifukwa chake, bFGF imawonedwa ngati chandamale cha njira zochiritsira za khansa ya chemopreventive ndi achire.

Ma cytokines
Expression Host	E.coli
Mawu ofanana	FGF2, FGFB, FGF zoyambira, HBGF-2
Kutsatizana kwa Mapuloteni	Katundu wa DNA wosunga FGF2(P09038) wamunthu adawonetsedwa popanda tag.
Misa ya Molecular	Imaneneratu kuchuluka kwa mamolekyulu a 17.1 kD.
Kuyesedwa kwa QC Kuyera	> 90 % malinga ndi SDS-PAGE.
Endotoxin
Zochita	Kuyesedwa mu kuyesa kwa kuchuluka kwa maselo pogwiritsa ntchito maselo a Balb/c 3T3. ED₅₀ chifukwa cha izi ndi ≤ 0.1ng / mL, yofanana ndi ntchito yapadera ya ≥ 1 x 10⁷IU / mg.
Kupanga	Lyophilized kuchokera wosabala PBS, pH 7.4. Nthawi zambiri 6 % mannitol amawonjezedwa ngati chitetezo pamaso lyophilization.
Kukhazikika	Miyezi 24 pa 2 ℃ mpaka 8 ℃ mu mkhalidwe wa lyophilized. Miyezi 6 pa -20 ℃ pansi pazikhalidwe zosabala pambuyo pa kukhazikitsidwanso. Miyezi 12 pa -80 ℃ pansi pazikhalidwe zosabala pambuyo pa kukhazikitsidwanso
Kusungirako	Ndibwino kuti aliquot mapuloteni muchuluke kakang'ono mutatha kukonzanso ndi madzi a jekeseni, saline wamba kapena PBS, ndikusunga ndende yosungunuka pamwamba pa 100μg/mL. Pewani kuzizira kobwerezabwereza.