Recombinant Human IL-2 mapuloteni

Stock: Mu Stock
Chithunzi cha GMP-TL906

Lumikizanani nafe

Kufotokozera

Ziwerengero za Data

Makhalidwe

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Maselo a T omwe atsegulidwa nthawi zambiri sangakhale ndi moyo kwanthawi yayitali mu chikhalidwe cha vitro, ndipo kuwonjezera kwa IL-2 kumatha kulimbikitsa kuchulukana kwawo kwanthawi yayitali komanso kosalekeza. Pamwamba pa maselo oima T safotokoza IL-2R ndipo samayankha IL-2; Maselo a T opangidwa ndi mitogen kapena zolimbikitsa zina zimatha kufotokoza IL-2R ndikukhala maselo omwe amawunikira a IL-2; IL-2 imathanso kupangitsa kuti IL-2R ichuluke m'maselo omwe akutsata. Mafotokozedwe a IL-2R pa ma T cell ndi osakhalitsa, nthawi zambiri amafika pachimake patatha masiku 2-3 atatsegulidwa ndikuzimiririka masiku 6-10. Ndi kutha kwa IL-2R, ma T cell amatha kuyankha IL-2. Chifukwa chake, kuti mukhalebe ndi kukula kwanthawi yayitali kwa maselo abwinobwino a T mu m'galasi, ndikofunikira kulimbikitsa ma T cell ndi mitogen kapena zolimbikitsa zina kuti asunge mawu a IL-2R. Maselo a NK ndi maselo okhawo a lymphoid omwe nthawi zambiri amawonetsa IL-2R, choncho nthawi zonse amakhala ndi reactivity ku IL-2. Komabe, IL-2R yokha imawonetsedwa pazitsulo za NK β β Chain ndi γ Unyolo uli ndi mgwirizano wochepa wa IL-2 ndipo ukhoza kuchitapo kanthu ndi IL-2. Ma cell a NK akatsegulidwa, amawonetsa IL-2R α Chain, kukhala cholandilira chogwirizana kwambiri.

Ma cytokines
Expression Host	E.coli
Mawu ofanana	TCGF, lymphokine, Interleukin 2
Misa ya Molecular	Mapuloteni ophatikizananso aumunthu a IL-2 amalosera kuchuluka kwa mamolekyulu a 15.4 kD.
Kuyesedwa kwa QC Kuyera	> 90 % malinga ndi SDS-PAGE.
Endotoxin
Zochita	Kutsimikiziridwa ndi kusonkhezera kodalira mlingo wa kuchuluka kwa maselo a CTLL-2 , zogwirizana ndi ntchito ya ≥1x10⁷U/mg.
Kupanga	Lyophilized kuchokera wosabala PBS, pH 7.4. Nthawi zambiri 6 % mannitol amawonjezedwa ngati chitetezo pamaso lyophilization.
Kukhazikika	Kukonzekera kwa Lyophilized kumatha kusungidwa pa 2-8 ℃. Miyezi 6 pa -20 ℃ pansi pazikhalidwe zosabala pambuyo pa kukhazikitsidwanso. Miyezi 12 pa -80 ℃ pansi pazikhalidwe zosabala pambuyo pa kukhazikitsidwanso.
Kusungirako	Ndibwino kuti aliquot mapuloteni muchuluke kakang'ono mutatha kukonzanso ndi madzi a jekeseni, saline wamba kapena PBS, ndikusunga ndende yosungunuka pamwamba pa 100μg/mL. Pewani kuzizira kobwerezabwereza.