Recombinant Human LIF mapuloteni

Stock: Mu Stock
Chithunzi cha GMP-TL633

Lumikizanani nafe

Kufotokozera

Makhalidwe

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Zowonetsa Zamalonda

Recombinant Human Leukemia Inhibitory Factor (LIF) ndi cytokine yogwira ntchito zambiri yomwe imagwira ntchito yofunikira pakuwongolera kusiyanasiyana kwa ma cell, kuchulukana, ndi kupulumuka. LIF imakhudzidwa ndi zochitika zosiyanasiyana za thupi, kuphatikizapo hematopoiesis, kutupa, ndi chitukuko cha embryonic. Puloteni iyi ndiyofunikira pakufufuza mu stem cell biology, immunology, ndi oncology.

Zofunika Kwambiri

1.Kuyera Kwambiri: Kuyeretsedwa pogwiritsa ntchito njira zapamwamba za chromatographic, mankhwalawa amakwaniritsa mulingo wachiyero wopitilira 95%, kuwonetsetsa kuti zoyeserera zolondola ndi zobwerezedwanso.

2.Ntchito Yapamwamba Yachilengedwe: Imatsimikiziridwa kudzera muzoyesa zingapo zantchito, puloteni ya LIF iyi imathandizira bwino kusiyanitsa kwa maselo ndi kupulumuka, ndikupangitsa kuti ikhale yoyenera pakugwiritsa ntchito kafukufuku wambiri.

3.Batch Consistency: Amapangidwa pansi pa njira zowongolera khalidwe labwino kuti zitsimikizire kusasinthasintha kwakukulu pakati pa magulu, kuchepetsa kusinthasintha ndi kupititsa patsogolo kudalirika koyesera.

4.Zopanda Zinyama: Zopangidwa popanda kugwiritsa ntchito zigawo zochokera ku zinyama, kutsata miyezo yopanda zinyama ndi kukulitsa kugwiritsidwa ntchito kwake pazofuna zosiyanasiyana zafukufuku.

Mapulogalamu

• Kafukufuku wa Stem Cell: Phunzirani ntchito ya LIF posunga kuchuluka kwa maselo amtundu wa embryonic ndikulimbikitsa kusiyana kwawo m'magulu osiyanasiyana a maselo, kupititsa patsogolo kafukufuku wamankhwala obwezeretsa.

• Immunology: Fufuzani zotsatira za LIF pakugwira ntchito kwa maselo a chitetezo cha mthupi ndi kusiyanitsa, kupereka zidziwitso za mayankho a chitetezo cha mthupi ndi ntchito zochiritsira zomwe zingatheke.

• Oncology: Onani ntchito ya LIF mu biology yama cell a khansa, kuphatikiza momwe imakhudzira kukula kwa chotupa ndi metastasis, zomwe zimathandizira pakupanga njira zatsopano zothandizira khansa.

• Hematopoiesis: Gwiritsani ntchito LIF kuti muphunzire momwe imagwirira ntchito pakuwongolera ma cell a hematopoietic stem cell ndi ma cell a progenitor cell, popereka chidziwitso chofunikira pochiza matenda a hematological.

Tsatanetsatane ndi Kuyika

• Zofotokozera: Zopezeka mumitundu yosiyanasiyana kuti zikwaniritse zofunikira zoyesera, kuphatikizapo 10μg, 50μg, 100μg, ndi 1mg.

• Kupaka: Amaperekedwa mu mawonekedwe a lyophilized kuti atsimikizire kukhazikika ndikuthandizira kusungirako ndi mayendedwe osavuta.

Kusungirako ndi Kukhazikika

• Kusungirako: Sungani pa -20°C kapena pansi kuti musunge bata ndi zochitika zamoyo. Pewani kuzizira kobwerezabwereza kuti mupewe kuwonongeka.

• Kukhazikika: Kukhazikika kwa kusungidwa kwa nthawi yayitali pansi pamikhalidwe yovomerezeka, kuonetsetsa kuti ntchito yodalirika ikugwira ntchito muzofukufuku.

Malangizo Ogwiritsa Ntchito

1.Sungani ufa wa lyophilized mu malo oyenera osabala m'malo osabala musanagwiritse ntchito.

2.Konzani ndikuchepetsani yankholo molingana ndi zofunikira zoyeserera kuti muwonetsetse zolondola komanso zobwereketsa.

3.Pewani kusakanikirana ndi zinthu zina zofananira kuti mupewe kuwoloka komanso kusokoneza komwe kungachitike.

Chenjezo

• Izi zimapangidwira kuti zigwiritsidwe ntchito pofufuza zokha ndipo sizoyenera kuwunikira matenda kapena chithandizo chamankhwala.

• Gwirani mankhwala molingana ndi malangizo omwe aperekedwa kuti musunge zomanga thupi komanso kukhulupirika.

• Pazovuta zilizonse kapena mafunso okhudzana ndi malonda, chonde lemberani gulu lathu laukadaulo.

Ma cytokines
Expression Host	HEK293 maselo
Mawu ofanana	CDF,DIA,HILDA,Leukemia Inhibitory Factor,MLPLI
Kutsatizana kwa Mapuloteni	DNA encoding human LIF(NP_002300.1) yowonetsedwa ndi tag Yake pa C-terminus.
Misa ya Molecular	Puloteni yophatikizanso ya LIF yamunthu imakhala ndi ma amino acid 186 ndipo imanenedweratu kuti imalemera 20.5kD.
Kuyesedwa kwa QC Kuyera	> 90 % malinga ndi SDS-PAGE.
Endotoxin	≤0.01EU/ug (njira ya gel osakaniza).
Zochita	Zimatsimikiziridwa ndi kukula kwa maselo a TF-1. ED₅₀ yomwe ikuyembekezeka pa izi ndi ≤ 0.1ng / mL, yogwirizana ndi ntchito yapadera ya ≥1 × 10⁷U/mg.
Kupanga
Kukhazikika	Miyezi 24 pa 4 ℃ mu mkhalidwe wa lyophilized. Miyezi 6-miyezi 12 pa -20 ℃ pansi pazikhalidwe zosabala pambuyo pa kukhazikitsidwanso. Pewani kuzizira kobwerezabwereza.
Kusungirako	Miyezi 24 pa 4 ℃ mu mkhalidwe wa lyophilized. Miyezi 6-miyezi 12 pa -20 ℃ pansi pazikhalidwe zosabala pambuyo pa kukhazikitsidwanso. Pewani kuzizira kobwerezabwereza.