Recombinant Human PDGF-BB Mapuloteni

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Chithunzi cha GMP-TL644

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Ma PDGF ndi ma dimers olumikizidwa ndi disulfide okhala ndi maunyolo awiri a 12.0-13.5 kDa polypeptide, osankhidwa PDGF-A ndi PDGF-B maunyolo. Ma PDGF atatu omwe amapezeka mwachilengedwe, PDGF-AA, PDGF-BB ndi PDGF-AB, ndi ma mitogens amphamvu amitundu yosiyanasiyana ya maselo, kuphatikizapo maselo osalala a minofu, maselo ogwirizana, mafupa ndi cartilage, ndi maselo ena a magazi. Ma PDGF amasungidwa m'mapulateleti α-granules, ndipo amamasulidwa akamatsegula mapulateleti. Ma PDGF amakhudzidwa ndi njira zingapo zachilengedwe, kuphatikiza hyperplasia, chemotaxis, embryonic neuron development, ndi kupuma kwa tubule epithelial cell. Ma receptor awiri odziwika bwino a PDGF adadziwika ndikutchedwa PDGFR-α ndi PDGFR-β. PDGFR-α ndi cholandilira chogwirizana kwambiri pamitundu itatu ya PDGF. Kumbali ina, PDGFR-β imalumikizana ndi PDGF-BB ndi PDGF-AB yokha.

Ma cytokines
Expression Host	HEK293 maselo
Mawu ofanana	PDGFBB(Platelet-Derived Growth Factor-BB), PDGFB, FLJ12858, PDGF2, SIS, SSV, c-sis Glioma-derived growth factor(GDGF), Osteosarcoma-derived Growth Factor(ODGF)
Kutsatizana kwa Mapuloteni	Kutsatizana kwa DNA kuyika PDGF-BB yamunthu (GenBank: CAA45383.1) idawonetsedwa ndi tag ya polyhistidine pa C-terminus.
Misa ya Molecular	Recombinant Human PDGF-BB imakhala ndi 115 amino acid ndipo imaneneratu kuchuluka kwa molekyulu ya 13.1 kDa.
Kuyesedwa kwa QC Kuyera	> 90 % malinga ndi SDS-PAGE.
Endotoxin
Zochita	Zimatsimikiziridwa ndi kukondoweza kumadalira mlingo wa kuchuluka kwa maselo a Balb/c 3T3. ED₅₀ yomwe ikuyembekezeka pa izi ndi ≤100ng/mL.
Kupanga	Lyophilized kuchokera wosabala PBS, pH 7.4. Nthawi zambiri 6% mannitol amawonjezedwa ngati chitetezo pamaso lyophilization.
Kukhazikika	Kukonzekera kwa Lyophilized kumatha kusungidwa pa -20 ℃. Miyezi 6 pa -20 ℃ pansi pazikhalidwe zosabala pambuyo pa kukhazikitsidwanso. Miyezi 12 pa -80 ℃ pansi pazikhalidwe zosabala pambuyo pa kukhazikitsidwanso.
Kusungirako	Ndibwino kuti aliquot mapuloteni muchuluke kakang'ono mutatha kukonzanso ndi madzi a jekeseni, saline wamba kapena PBS, ndikusunga ndende yosungunuka pamwamba pa 100μg/mL. Pewani kuzizira kobwerezabwereza.