Recombinant Human SCF mapuloteni

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Stem Cell Factor, yomwe imadziwikanso kuti SCF, kit-ligand, KL, steel factor, KITLG, FPH2, KL-1, Kitl, MGF, SCF, SF, kapena SHEP7, ndi molekyulu ya dimeric yomwe imagwira ntchito zake zachilengedwe pomanga ndi kuyambitsa receptor tyrosine kinase c-Kit. Kutsegula kwa c-Kit kumabweretsa autophosphorylation yake ndikuyambitsa kutulutsa ma siginecha. Mapuloteni owonetsera amalembedwa kuti alowetse c-Kit ndi madera ena ogwirizana omwe amamangiriza zotsalira za phosphorylated tyrosine m'chigawo cha intracellular cha c-Kit. Kumanga kwa SCF ku C-kit kumapangitsa kuti receptor dimerization ndi autophosphorylation ya zotsalira za tyrosine mu cytoplasmic domain. Tyrosine phosphorylation imayambitsa njira zingapo zowonetsera kuphatikiza RAS, PI3 kinase, Src, ndi JAK/STAT. Komabe, SCF ndiyomwe imathandizira kusiyanitsa mitundu yambiri ya maselo monga ma spermatogonial stem cell ndi megakaryocyte progenitors. Kupatula kusiyanitsa, SCF imathanso kusunga kukhazikika m'maselo. Pazachipatala, SCF imagwiritsidwa ntchito limodzi ndi ma cytokines ena kupanga maselo opondereza opangidwa ndi myeloid kuchokera kumagazi amunthu. SCF imagwiritsidwanso ntchito popanga ma T cell othandizira ma cell, kuyesa mankhwala ndi kutengera matenda. M'maphunziro obwezeretsanso, SCF imagwiritsidwa ntchito pochiritsa mabala hydrogel ngati njira yowonjezerera mphamvu yake yomatira ndi kusinthika kwa minofu.

Ma cytokines
Expression Host	HEK293 maselo
Mawu ofanana	C-kitligand,DCUA,FPH2,FPHH,Kitl,KL-1,MGF,SCF,SF,SHEP7
Kutsatizana kwa Mapuloteni	Katundu wa DNA woyika SCF yamunthu (NP_000890.1) adawonetsedwa ndi chizindikiro Chake pa C-terminus.
Misa ya Molecular	Mapuloteni ophatikizananso a SCF amunthu amakhala ndi ma amino acid 170 ndipo amalosera kuchuluka kwa mamolekyulu a 19.3 kD.
Kuyesedwa kwa QC Kuyera	> 90 % malinga ndi SDS-PAGE.
Endotoxin
Zochita	Kuyesedwa mu kuyesa kwa kuchuluka kwa maselo pogwiritsa ntchito maselo a Mo7e, ofanana ndi ntchito ya ≥2.0x10⁵IU/mg.
Kupanga	Lyophilized kuchokera wosabala PBS, pH 7.4. Nthawi zambiri 6 % mannitol amawonjezedwa ngati chitetezo pamaso lyophilization.
Kukhazikika	Kukonzekera kwa Lyophilized kumatha kusungidwa pa -20 ℃. Miyezi 6 pa -20 ℃ pansi pazikhalidwe zosabala pambuyo pa kukhazikitsidwanso. Miyezi 12 pa -80 ℃ pansi pazikhalidwe zosabala pambuyo pa kukhazikitsidwanso.
Kusungirako	Ndibwino kuti aliquot mapuloteni muchuluke kakang'ono mutatha kukonzanso ndi madzi a jekeseni, saline wamba kapena PBS, ndikusunga ndende yosungunuka pamwamba pa 100μg/mL. Pewani kuzizira kobwerezabwereza.