0102030405
Recombinant Human VEGF165 mapuloteni
Zowonetsa Zamalonda
Recombinant Human Transforming Growth Factor Beta 3 (TGF-β3) ndi cytokine yambiri yomwe imakhudzidwa ndi kayendetsedwe ka kukula kwa maselo, kusiyanitsa, ndi mayankho a chitetezo cha mthupi. Mwa kumangiriza ku zolandilira zake, TGF-β3 imayendetsa njira zowonetsera zotsika zomwe zimayang'anira kupanga matrix owonjezera ndi kukonza minofu, ndikupangitsa kuti ikhale chida chofunikira pakufufuza kwa ma cell biology.
Zofunika Kwambiri
1.Kuyera Kwambiri: Kuyeretsedwa pogwiritsa ntchito njira zapamwamba za chromatographic, mankhwalawa amakwaniritsa mulingo wachiyero wopitilira 95%, kuwonetsetsa kuti zoyeserera zolondola ndi zobwerezedwanso.
2.Ntchito Yapamwamba Yachilengedwe: Kutsimikiziridwa kudzera m'mayesero angapo a ntchito, puloteni iyi ya VEGF165 imapangitsa kuti maselo a endothelial achuluke komanso kusamuka, ndikupangitsa kuti ikhale yoyenera pazofukufuku zosiyanasiyana.
3.Batch Consistency: Amapangidwa pansi pa njira zowongolera khalidwe labwino kuti zitsimikizire kusasinthasintha kwakukulu pakati pa magulu, kuchepetsa kusinthasintha ndi kupititsa patsogolo kudalirika koyesera.
4.Zopanda Zinyama: Zopangidwa popanda kugwiritsa ntchito zigawo zochokera ku zinyama, kutsata miyezo yopanda zinyama ndi kukulitsa kugwiritsidwa ntchito kwake pazofuna zosiyanasiyana zafukufuku.
Mapulogalamu
• Oncology: Phunzirani ntchito ya VEGF165 mu chotupa cha angiogenesis, kupereka chidziwitso cha kukula kwa khansa ndi chitukuko cha mankhwala odana ndi angiogenic.
• Kafukufuku wamtima: Fufuzani zotsatira za VEGF165 pa thanzi la mitsempha ndi zomwe zingatheke pochiza matenda a mtima, kuphatikizapo ischemic.
• Umisiri wa Tissue: Gwiritsani ntchito VEGF165 popanga minyewa yopangidwa mwaluso ndi ziwalo zolimbikitsira vascularization ndi minyewa.
• Kuchiritsa Mabala: Fufuzani ntchito ya VEGF165 mu njira zochiritsira mabala, kuphatikizapo kukonzanso minofu ndi kusinthika, kupereka njira zochiritsira zomwe zingatheke.
Tsatanetsatane ndi Kuyika
• Zofotokozera: Zopezeka mumitundu yosiyanasiyana kuti zikwaniritse zofunikira zoyesera, kuphatikizapo 10μg, 50μg, 100μg, ndi 1mg.
• Kupaka: Amaperekedwa mu mawonekedwe a lyophilized kuti atsimikizire kukhazikika ndikuthandizira kusungirako ndi mayendedwe osavuta.
Kusungirako ndi Kukhazikika
• Kusungirako: Sungani pa -20°C kapena pansi kuti musunge bata ndi zochitika zamoyo. Pewani kuzizira kobwerezabwereza kuti mupewe kuwonongeka.
• Kukhazikika: Kukhazikika kwa kusungidwa kwa nthawi yayitali pansi pamikhalidwe yovomerezeka, kuonetsetsa kuti ntchito yodalirika ikugwira ntchito muzofukufuku.
Malangizo Ogwiritsa Ntchito
1.Sungani ufa wa lyophilized mu malo oyenera osabala m'malo osabala musanagwiritse ntchito.
2.Konzani ndikuchepetsani yankho molingana ndi zofunikira zoyeserera kuti mukwaniritse zolondola komanso zobwereketsa.
3.Pewani kusakanikirana ndi zinthu zina zofananira kuti mupewe kuwoloka komanso kusokoneza komwe kungachitike.
Chenjezo
• Izi zimapangidwira kuti zigwiritsidwe ntchito pofufuza zokha ndipo sizoyenera kuwunikira matenda kapena chithandizo chamankhwala.
• Gwirani mankhwala molingana ndi malangizo omwe aperekedwa kuti mukhale ndi mapuloteni komanso kukhulupirika.
• Pazovuta zilizonse kapena mafunso okhudzana ndi malonda, chonde lemberani gulu lathu laukadaulo.
| Ma cytokines | |
| Expression Host | Maselo a HEK293 |
| Mawu ofanana | MVCD1,VEGF,VEGF165,VPF |
| Kutsatizana kwa Mapuloteni | Kutsatira kwa DNA komwe kumayika VEGF165 yamunthu (NP_001165097.1) kudawonetsedwa ndi tag ya polyhistidine pa C-terminus. |
| Misa ya Molecular | Recombinant Human VEGF165 imakhala ndi 193 amino acid ndipo imaneneratu kuchuluka kwa molekyulu ya 22.3 kDa. |
| Kuyesedwa kwa QC Kuyera | > 90 % malinga ndi SDS-PAGE. |
| Endotoxin | |
| Zochita | Zimatsimikiziridwa ndi kukondoweza kumadalira mlingo wa kuchuluka kwa anthu umbilical vein endothelial cell (HUVEC). ED₅₀ yomwe ikuyembekezeka pa izi ndi ≤20 ng/mL. |
| Kupanga | Lyophilized kuchokera wosabala PBS, pH 7.4. Nthawi zambiri 6% mannitol amawonjezedwa ngati chitetezo pamaso lyophilization. |
| Kukhazikika | Miyezi 24 pa 2 ℃ mpaka 8 ℃ mu mkhalidwe wa lyophilized. Miyezi 6 pa -20 ℃ pansi pazikhalidwe zosabala pambuyo pa kukhazikitsidwanso. Miyezi 12 pa -80 ℃ pansi pazikhalidwe zosabala pambuyo pa kukhazikitsidwanso. |
| Kusungirako | Ndibwino kuti aliquot mapuloteni muchuluke kakang'ono mutatha kukonzanso ndi madzi a jekeseni, saline wamba kapena PBS, ndikusunga ndende yosungunuka pamwamba pa 100μg/mL. Pewani kuzizira kobwerezabwereza. |
| Chala chachikulu | Zambiri zamafayilo |
 | GMP-TL612_Product Sheet.pdf |
| Chithunzi cha GMP-TL612_SDS |