Recombinant Human Fibronectin(FN) Protein

Stock: In Stock
Model: GMP-TL903

Description

Data Figures

Characterization

Document Files

Product Overview

Recombinant Human Fibronectin (FN) is a vital extracellular matrix (ECM) protein that plays a critical role in cell adhesion, migration, and tissue repair. Fibronectin interacts with various cellular receptors, including integrins, to influence cell behavior and promote tissue integrity. This recombinant protein is an essential tool for research in cell biology, tissue engineering, and regenerative medicine.

Key Features

1.High Purity: This product is purified using advanced chromatographic techniques, achieving a purity level of over 95%, ensuring accurate and reproducible experimental outcomes.

2.High Biological Activity: Demonstrated through multiple activity assays, this product effectively supports cell adhesion, migration, and proliferation, making it suitable for various cell culture applications.

3.Batch Consistency: Produced under stringent quality control measures to ensure consistency between batches, minimizing variability and enhancing the reliability of research results.

4.Animal-Free: Manufactured without the use of animal-derived components, adhering to animal-free standards and expanding its applicability for diverse research needs.

Applications

• Cell Adhesion Studies: Investigate the role of fibronectin in cell adhesion and migration, contributing to a deeper understanding of cell-matrix interactions.

• Tissue Engineering: Utilize fibronectin in tissue scaffolds and matrices to promote cell attachment and tissue regeneration, advancing the development of regenerative therapies.

• Wound Healing Research: Explore the impact of fibronectin on wound healing processes, including tissue repair and remodeling, providing insights into potential therapeutic approaches.

• Cancer Research: Study the role of fibronectin in tumor cell behavior and metastasis, offering valuable information for cancer research and treatment strategies.

Specifications and Packaging

• Specifications: Available in various sizes to accommodate different experimental requirements, including 10μg, 50μg, 100μg, and 1mg.

• Packaging: Supplied in sterile lyophilized form to facilitate storage and transportation.

Storage and Stability

• Storage: Store at -20°C or below to maintain product stability and biological activity. Avoid repeated freeze-thaw cycles to prevent degradation.

• Stability: Remains stable under recommended storage conditions, ensuring long-term usability and reliability in research applications.

Instructions for Use

1.Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment prior to use.

2.Prepare and dilute the solution according to experimental requirements for accurate and reproducible results.

3.Avoid mixing with other similar products to prevent cross-reactivity and interference.

Cautions

• This product is intended for research use only and is not suitable for clinical diagnostics or therapeutic applications.

• Follow handling instructions carefully to preserve protein activity and integrity.

• For any issues or inquiries regarding the product, please contact our technical support team.

Cytokines
Expression Host	HEK293 cells
Synonym	FLT3LG,FL,FLT3L,Flt3 ligand
Protein Sequence	A DNA sequence encoding the human FLT3 Ligand(NP_001450.2）was expressed with a His tag at the C-terminus.
Molecular Mass	The recombinant human FLT3 Ligand protein contains 161 amino acids with a predicted molecular weight of 18.4 kD.
QC Testing Purity	> 90 % as determined by SDS-PAGE.
Endotoxin	< 0.1EU per 1μg of the protein as determined by the LAL method.
Activity	Measured by the dose-dependent stimulation of the proliferation of OCI-AML5 cells.The specific activity is ≥5.0×10⁵IU/mg.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 6 % mannitol are added as protectants before lyophilization.
Stability	Lyophilized preparation can be stored at -20℃. 6 months at -20℃ under sterile conditions after reconstitution. 12 months at -80℃ under sterile conditions after reconstitution.
Storage	Recommend to aliquot the protein into smaller quantities after reconstituting with water for injection, normal saline or PBS, and keep the diluted concentration above 100μg/mL. Avoid repeated freeze-thaw cycles.