Recombinant Human FLT3 Ligand Protein

Stock: In Stock
Model: GMP-TL505

Description

Data Figures

Characterization

Document Files

Product Overview

Recombinant Human FLT3 Ligand (FLT3L) is a vital cytokine involved in the regulation of hematopoietic stem cell growth and differentiation. FLT3L binds to the FLT3 receptor on hematopoietic cells, promoting their proliferation and survival. This protein is essential for research in hematology, immunology, and cancer biology, providing valuable insights into cell development and immune response.

Key Features

1.High Purity: This product is purified through advanced chromatographic techniques, achieving a purity level of over 95%, ensuring accurate and consistent experimental results.

2.High Biological Activity: Demonstrated through multiple activity assays, this protein effectively stimulates FLT3 receptor-positive cells, supporting research on cell proliferation and differentiation.

3.Batch Consistency: Produced with stringent quality control measures to ensure high consistency between batches, reducing variability and enhancing the reliability of research outcomes.

4.Animal-Free: Manufactured without the use of animal-derived components, adhering to animal-free standards and broadening its application scope for diverse research needs.

Applications

• Hematology: Study the role of FLT3L in the growth and differentiation of hematopoietic stem cells and progenitor cells, providing insights into blood cell development and potential treatments for hematological disorders.

• Immunology: Investigate the effects of FLT3L on immune cell development and function, exploring its potential in immunotherapy and immune system modulation.

• Cancer Research: Examine the role of FLT3L in cancer cell biology, including tumor growth and immune cell interactions, contributing to the development of novel cancer therapies.

• Stem Cell Research: Utilize FLT3L to support the expansion and maintenance of stem cells in culture, advancing research in regenerative medicine and cell-based therapies.

Specifications and Packaging

• Specifications: Available in various sizes to meet different experimental requirements, including 10μg, 50μg, 100μg, and 1mg.

• Packaging: Supplied in sterile lyophilized form to facilitate storage and transportation.

Storage and Stability

• Storage: Store at -20°C or below to maintain product stability and biological activity. Avoid repeated freeze-thaw cycles to prevent degradation.

• Stability: Stable for long-term storage under recommended conditions, ensuring reliable performance in research applications.

Instructions for Use

1.Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment prior to use.

2.Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.

3.Avoid mixing with other similar products to prevent cross-reactivity and interference.

Cautions

• This product is intended for research use only and is not suitable for clinical diagnostics or therapeutic applications.

• Follow handling instructions carefully to preserve protein activity and integrity.

• For any issues or inquiries regarding the product, please contact our technical support team.

Cytokines
Expression Host	HEK293 cells
Synonym	FLT3LG,FL,FLT3L,Flt3 ligand
Protein Sequence	A DNA sequence encoding the human FLT3 Ligand(NP_001450.2）was expressed with a His tag at the C-terminus.
Molecular Mass	The recombinant human FLT3 Ligand protein contains 161 amino acids with a predicted molecular weight of 18.4 kD.
QC Testing Purity	> 90 % as determined by SDS-PAGE.
Endotoxin	< 0.1EU per 1μg of the protein as determined by the LAL method.
Activity	Measured by the dose-dependent stimulation of the proliferation of OCI-AML5 cells.The specific activity is ≥5.0×10⁵IU/mg.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 6 % mannitol are added as protectants before lyophilization.
Stability	Lyophilized preparation can be stored at -20℃. 6 months at -20℃ under sterile conditions after reconstitution. 12 months at -80℃ under sterile conditions after reconstitution.
Storage	Recommend to aliquot the protein into smaller quantities after reconstituting with water for injection, normal saline or PBS, and keep the diluted concentration above 100μg/mL. Avoid repeated freeze-thaw cycles.