Recombinant Human GM-CSF Protein

Stock: In Stock
Model: GMP-TL302

Description

Data Figures

Characterization

Document Files

Product Overview

Recombinant Human Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) is a critical cytokine that plays a significant role in the regulation of hematopoiesis and immune responses. GM-CSF promotes the differentiation, proliferation, and activation of granulocyte and macrophage precursor cells, enhancing their functions. This protein is essential for research in immunology, hematology, and cancer therapy.

Key Features

1.High Purity: Purified using advanced chromatographic techniques, this product achieves a purity level of over 95%, ensuring high reliability and reproducibility of experimental results.

2.High Biological Activity: Validated through multiple activity assays, this GM-CSF protein effectively stimulates target cells, supporting a variety of research applications related to immune cell function and hematopoiesis.

3.Batch Consistency: Produced with strict quality control measures to ensure consistent quality between batches, minimizing variability and enhancing the reliability of research outcomes.

4.Animal-Free: Manufactured without animal-derived components, complying with animal-free standards and broadening its applicability for various research needs.

Applications

• Hematology: Study the role of GM-CSF in the regulation of blood cell production, including granulocytes and macrophages, providing insights into hematopoietic disorders and therapies.

• Immunology: Investigate GM-CSF’s effects on immune cell development and activation, contributing to a deeper understanding of immune responses and potential therapeutic applications.

• Cancer Research: Examine the role of GM-CSF in tumor microenvironments and its impact on immune cell function, offering insights into cancer immunotherapy and treatment strategies.

• Cell Therapy: Utilize GM-CSF to enhance the function and expansion of immune cells in cell-based therapies, advancing research in regenerative medicine and immunotherapy.

Specifications and Packaging

• Specifications: Available in various sizes to accommodate different experimental requirements, including 10μg, 50μg, 100μg, and 1mg.

• Packaging: Supplied in sterile lyophilized form to ensure stability and facilitate convenient storage and transportation.

Storage and Stability

• Storage: Store at -20°C or below to maintain protein stability and biological activity. Avoid repeated freeze-thaw cycles to prevent degradation.

• Stability: Remains stable for long-term storage under recommended conditions, ensuring reliable performance in research applications.

Instructions for Use

1.Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.

2.Prepare and dilute the solution according to experimental requirements to achieve accurate and reproducible results.

3.Avoid mixing with other similar products to prevent cross-reactivity and potential interference.

Cautions

• This product is intended for research use only and is not suitable for clinical diagnostics or therapeutic applications.

• Handle the product according to provided instructions to maintain protein activity and integrity.

• For any issues or inquiries regarding the product, please contact our technical support team.

Cytokines
Expression Host	HEK293 cells
Synonym	GM-CSF,CSF2,MGC131935
Protein Sequence	NP_000749.2：A18-E144 was expressed with a His-tag at the C-terminus.
Molecular Mass	Predicts a molecular mass of 15.3kD.
QC Testing Purity	> 90 % as determined by SDS-PAGE.
Endotoxin	< 0.1EU per 1 μg of the protein by the LAL method.
Activity	Measured by the dose-dependent stimulation of the proliferation of TF-1 cells，the specific activity is ≥5.0×10⁶IU/mg.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 6 % mannitol are added as protectants before lyophilization.
Stability	Lyophilized preparation can be stored at -20 ℃. 6 months at -20℃ under sterile conditions after reconstitution. 12 months at -80℃ under sterile conditions after reconstitution.
Storage	Recommend to aliquot the protein into smaller quantities after reconstituting with water for injection, normal saline or PBS, and keep the diluted concentration above 100μg/mL. Avoid repeated freeze-thaw cycles.