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Recombinant Human IFN-γ Protein
Product Overview
Recombinant Human Interferon Alpha (IFN-α) is a key cytokine in the regulation of immune responses, known for its antiviral, antiproliferative, and immunomodulatory properties. IFN-α is critical in the defense against viral infections and the modulation of immune responses in various diseases. This recombinant protein is essential for research in virology, immunology, and oncology.
Key Features
1.High Purity: This product is purified using advanced chromatographic techniques, achieving a purity level of over 95%, ensuring reliable and reproducible results in experiments.
2.High Biological Activity: Confirmed through multiple activity assays, this IFN-α protein effectively induces antiviral and antiproliferative effects in target cells, making it suitable for a range of research applications.
3.Batch Consistency: Produced with rigorous quality control measures to ensure consistent quality between batches, reducing variability and enhancing experimental reliability.
4.Animal-Free: Manufactured without animal-derived components, adhering to animal-free standards and broadening its applicability for various research purposes.
Applications
• Virology: Study the antiviral mechanisms of IFN-α, providing insights into its role in controlling viral infections and developing antiviral therapies.
• Immunology: Investigate the immunomodulatory effects of IFN-α on immune cell functions and interactions, contributing to a better understanding of immune responses and potential treatments for immune disorders.
• Oncology: Explore the antiproliferative effects of IFN-α on cancer cells, offering valuable information for the development of cancer immunotherapies and treatment strategies.
• Cell Culture: Utilize IFN-α to enhance cell culture systems, providing a more controlled environment for studying cellular responses and drug effects.
Specifications and Packaging
• Specifications: Available in various sizes to meet different experimental needs, including 10μg, 50μg, 100μg, and 1mg.
• Packaging: Supplied in sterile lyophilized form to facilitate storage and transport.
Storage and Stability
• Storage: Store at -20°C or below to maintain product stability and biological activity. Avoid repeated freeze-thaw cycles to prevent degradation.
• Stability: Stable for long-term storage under recommended conditions, ensuring reliable performance in research applications.
Instructions for Use
1.Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
2.Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
3.Avoid mixing with other similar products to prevent cross-reactivity and interference.
Cautions
• This product is intended for research use only and is not suitable for clinical diagnostics or therapeutic applications.
• Handle the product according to provided instructions to preserve protein activity and integrity.
• For any issues or inquiries regarding the product, please contact our technical support team.
| Cytokines | |
| Expression Host | HEK293 cells |
| Synonym | Interferon-gamma, Interferon-γ |
| Protein Sequence | Accession # AAH70256 Q24-Q166 |
| Molecular Mass | Predicts a molecular mass of 17.6 kDa |
| QC Testing Purity | > 90 % as determined by SDS-PAGE |
| Endotoxin | < 0.1EU per 1 μg of the protein by the LAL method. |
| Activity | Measured in anti-viral assays protecting human amniotic cells (WISH) from the destructive effects of vesicular stomatitis virus (VSV). The ED₅₀ for this effect is ≤50 ng/mL. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. Normally 6 % mannitol are added as protectants before lyophilization. |
| Stability | 24 months at -20℃ in lyophilized state. 6 months at -20℃ under sterile conditions after reconstitution. 12 months at -80℃ under sterile conditions after reconstitution. |
| Storage | Recommend to aliquot the protein into smaller quantities after reconstituting with water for injection, normal saline or PBS, and keep the diluted concentration above 100μg/mL. Avoid repeated freeze-thaw cycles. |
| Thumb | File information |
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GMP-TL105_Product Sheet.pdf |
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GMP-TL105_SDS.pdf |