Recombinant Human IL-12 Protein

Stock: In Stock
Model: GMP-TL508

Description

Data Figures

Characterization

Document Files

Product Overview

Recombinant Human Interleukin-12 (IL-12) is a potent cytokine composed of two subunits, p35 and p40, that plays a crucial role in the regulation of immune responses. IL-12 is pivotal in promoting the differentiation of naive T cells into Th1 cells, enhancing the cytotoxic activity of natural killer (NK) cells, and stimulating the production of interferon-gamma (IFN-γ). This protein is essential for research in immunology, oncology, and infectious diseases.

Key Features

1.High Purity: Purified using advanced chromatographic techniques, this product achieves a purity level of over 95%, ensuring accurate and reproducible experimental results.

2.High Biological Activity: Validated through multiple activity assays, this IL-12 protein effectively promotes T cell differentiation and NK cell activity, making it suitable for a range of research applications.

3.Batch Consistency: Produced under stringent quality control measures to ensure high consistency between batches, reducing variability and enhancing experimental reliability.

4.Animal-Free: Manufactured without the use of animal-derived components, adhering to animal-free standards and broadening its applicability for diverse research needs.

Applications

• Immunology Research: Study the role of IL-12 in the regulation of immune responses, including the differentiation of T cells and the activation of NK cells, providing insights into immune mechanisms and potential therapeutic targets.

• Oncology: Explore the effects of IL-12 on tumor immunity, including its ability to enhance the cytotoxic activity of immune cells against cancer cells, contributing to the development of immunotherapeutic approaches.

• Infectious Diseases: Investigate the role of IL-12 in host defense mechanisms against infections, offering valuable information for the development of new treatments for infectious diseases.

• Vaccine Development: Utilize IL-12 in the design and testing of vaccines to enhance immune responses and improve vaccine efficacy.

Specifications and Packaging

• Specifications: Available in various sizes to meet different experimental requirements, including 10μg, 50μg, 100μg, and 1mg.

• Packaging: Supplied in sterile lyophilized form to ensure stability and facilitate convenient storage and transportation.

Storage and Stability

• Storage: Store at -20°C or below to maintain product stability and biological activity. Avoid repeated freeze-thaw cycles to prevent degradation.

• Stability: Stable for long-term storage under recommended conditions, ensuring reliable performance in research applications.

Instructions for Use

1.Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.

2.Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.

3.Avoid mixing with other similar products to prevent cross-reactivity and potential interference.

Cautions

• This product is intended for research use only and is not suitable for clinical diagnostics or therapeutic applications.

• Handle the product according to provided instructions to preserve protein activity and integrity.

• For any issues or inquiries regarding the product, please contact our technical support team.

Cytokines
Expression Host	HEK293 cells
Synonym	IL12, p70, Interleukin-12
Protein Sequence	Recombinant human IL-12 is heterodimeric and composed of P29459-1 (IL12A) and P29460-1 (IL12B).
Molecular Mass	The Recombinant Human IL-12 Protein predicts a molecular mass of 65 kDa.
QC Testing Purity	> 90 % as determined by SDS-PAGE.
Endotoxin	< 0.1EU per 1 μg of the protein by the LAL method.
Activity	Measured by cell proliferation assay on PHA activated PBMC cells.The ED50 of this effect is ≤ 0.2 ng/mL.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 6 % mannitol are added as protectants before lyophilization.
Stability	24 months at 2℃ to 8℃ in lyophilized state. 6 months at -20℃ under sterile conditions after reconstitution. 12 months at -80℃ under sterile conditions after reconstitution.
Storage	Recommend to aliquot the protein into smaller quantities after reconstituting with water for injection, normal saline or PBS, and keep the diluted concentration above 100μg/mL. Avoid repeated freeze-thaw cycles.