Recombinant Human IL-6 Protein

Stock: In Stock
Model: GMP-TL512

Description

Data Figures

Characterization

Document Files

Product Overview

Recombinant Human Interleukin-6 (IL-6) is a multifunctional cytokine that plays a pivotal role in immune response, inflammation, and hematopoiesis. IL-6 influences various cellular processes by binding to its receptor, IL-6R, and activating downstream signaling pathways. This makes it an essential tool for research in immunology, oncology, and cell biology.

Key Features

1.High Purity: This product undergoes stringent purification processes, achieving a purity level of over 95%, ensuring the reliability and reproducibility of experimental results.

2.High Biological Activity: Verified through multiple activity assays, this product effectively induces the expected biological responses in target cells, suitable for a wide range of research applications.

3.Batch Consistency: Manufactured under strict production and quality control protocols to ensure high consistency between batches, reducing variability in experimental outcomes.

4.Animal-Free: Produced without the use of animal-derived components, meeting animal-free requirements and broadening its application scope in research.

Applications

• Immunology: Study the role of IL-6 in immune responses, inflammation, and autoimmune diseases, providing insights into potential therapeutic targets.

• Oncology: Investigate the effects of IL-6 on tumor cells, tumor microenvironment, and cancer therapy, aiding in the development of novel anti-cancer strategies.

• Hematopoiesis: Explore the role of IL-6 in the regulation of blood cell production and its implications for treating hematological disorders.

• Cell Biology: Research the impact of IL-6 on cell signaling pathways, apoptosis, and cellular stress responses, contributing to a deeper understanding of cell biology.

Specifications and Packaging

• Specifications: Available in various sizes to meet different experimental needs, including 10μg, 50μg, 100μg, and 1mg.

• Packaging: Supplied in sterile lyophilized form for ease of storage and transport.

Storage and Stability

• Storage: Store at -20°C or below to maintain product stability and activity. Avoid repeated freeze-thaw cycles to prevent protein degradation.

• Stability: Stable for long-term storage under recommended conditions, ensuring reliable performance in research applications.

Instructions for Use

1.Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.

2.Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.

3.Do not mix with other similar products to avoid cross-reactivity and potential interference.

Cautions

• This product is intended for research use only and is not suitable for clinical diagnostics or therapeutic applications.

• Handle the product according to provided instructions to preserve its activity and stability.

• Contact our technical support team if you encounter any issues or have questions regarding the product's use.

Cytokines
Expression Host	HEK293 cells
Protein Sequence	A DNA sequence encoding the human IL-6（NP_000591.1：V30-M212）was expressed with a His-tag at the C-terminus.
Molecular Mass	The Recombinant Human IL-6 Protein predicts a molecular mass of 21.6 kD.
QC Testing Purity	> 90 % as determined by SDS-PAGE
Endotoxin	< 0.1EU per μg of the protein as determined by the LAL method.
Activity	Determined by the dose-dependent stimulation of the proliferation of TF-1 cells，the activity is ≥2.0×10 7 U/mg.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 6 % mannitol are added as protectants before lyophilization.
Stability	24 months at 2℃ to 8℃ in lyophilized state. 6 months at -20℃ under sterile conditions after reconstitution. 12 months at -80℃ under sterile conditions after reconstitution.
Storage	Recommend to aliquot the protein into smaller quantities after reconstituting with water for injection, normal saline or PBS, and keep the diluted concentration above 100μg/mL. Avoid repeated freeze-thaw cycles.