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Recombinant Human TNFα Protein
Product Overview
Recombinant Human TNF-α Protein is a bioactive cytokine involved in systemic inflammation and is a member of the TNF superfamily. This protein is critical for research applications focusing on immune response, inflammation, and apoptosis.
Key Features
● High Biological Activity: Maintains high bioactivity, essential for accurate experimental outcomes.
● Purity: Produced with high purity, ensuring minimal contamination and reliable results.
● Recombinant Production: Expressed in a controlled environment to ensure consistency and reproducibility.
● Versatile Applications: Suitable for various research applications, including cell signaling, cancer research, and immunology.
Applications
● Inflammation Research: Ideal for studying the mechanisms of inflammation and related diseases.
● Cancer Studies: Useful in research on tumor necrosis and the role of TNF-α in cancer.
● Cell Signaling: Facilitates research on TNF-α signaling pathways and their biological effects.
Specifications and Packaging
• Specifications: Available in various sizes to meet different experimental needs, including 10μg, 50μg, 100μg, and 1mg.
• Packaging: Supplied in sterile lyophilized form for ease of storage and transport.
Storage and Stability
• Storage: Store at -20°C or below to maintain product stability and activity. Avoid repeated freeze-thaw cycles to prevent protein degradation.
• Stability: Stable for long-term storage under recommended conditions, ensuring reliable performance in research applications.
Instructions for Use
1.Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
2.Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
3.Do not mix with other similar products to avoid cross-reactivity and potential interference.
Cautions
• This product is intended for research use only and is not suitable for clinical diagnostics or therapeutic applications.
• Handle the product according to provided instructions to preserve its activity and stability.
• Contact our technical support team if you encounter any issues or have questions regarding the product's use.
| Cytokines | |
| Expression Host | HEK293 cells |
| Synonym | DIF,TNF-alpha,TNFA,TNFSF2,cachexin,cachectin,TNFα |
| Protein Sequence | A DNA sequence encoding the human TNF-α (GenBank: ADV31546.2 V77-L233) was expressed with a polyhistidine tag at the C- terminus. |
| Molecular Mass | The Recombinant protein predicts a molecular mass of 18.2kDa. |
| QC Testing Purity | > 90% as determined by SDS-PAGE. |
| Endotoxin | < 0.1EU per μg of the protein as determined by the LAL method. |
| Activity | Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of actinomycin D. The ED₅₀ for this effect is ≤5ng/mL. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. Normally 6 % mannitol are added as protectants before lyophilization. |
| Stability | 24 months at 2℃ to 8℃ in lyophilized state. 6 months at -20℃ under sterile conditions after reconstitution. 12 months at -80℃ under sterile conditions after reconstitution. |
| Storage | Recommend to aliquot the protein into smaller quantities after reconstituting with water for injection, normal saline or PBS, and keep the diluted concentration above 100μg/mL. Avoid repeated freeze-thaw cycles. |
| Thumb | File information |
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GMP-TL303_SDS.pdf |
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GMP-TL303_Product Sheet.pdf |