Recombinant Human PDGF-BB Porotini

Fa'asoa: O lo'o iai Fa'asoa
Fa'ata'ita'iga: GMP-TL644

Faafesootai matou

Fa'amatalaga

Fa'amaumauga Fa'amaumauga

Fa'ailoga

Faila o Pepa

PDGFs o disulfide-linked dimers e aofia ai le lua 12.0-13.5 kDa polypeptide filifili, filifilia PDGF-A ma PDGF-B filifili. O PDGF e tolu, PDGF-AA, PDGF-BB ma le PDGF-AB, o mitogens malosi mo ituaiga eseese o sela, e aofia ai sela maso lamolemole, sela o mea e fesoʻotaʻi, ponaivi ma sela o le cartilage, ma nisi sela toto. O PDGFs o loʻo teuina i platelet α-granules, ma faʻasaʻoloto i luga o le faʻagaioiina o platelet. O le PDGFs o loʻo aʻafia i le tele o faʻagasologa o meaola, e aofia ai le hyperplasia, chemotaxis, atinaʻe o le embryonic neuron, ma le atinaʻeina o sela epithelial tubule respiratory. E lua fa'ailoga fa'ailoga ma'oti o PDGF ua fa'ailoa ma fa'aigoaina PDGFR-α ma le PDGFR-β. O le PDGFR-α o se faʻamaufaʻailoga maualuga mo le tolu PDGF fomu. I le isi itu, o le PDGFR-β e fegalegaleai naʻo le PDGF-BB ma le PDGF-AB.

Cytokines
Fa'aaliga Host	HEK293 sela
upu uiga tutusa	PDGFBB(Platelet-Derived Growth Factor-BB), PDGFB, FLJ12858, PDGF2, SIS, SSV, c-sis Glioma-fa'atupuina fa'atuputupu a'e (GDGF), Osteosarcoma-mafuaina Growth Factor(ODGF)
Polotini Fa'asologa	O se DNA faʻasologa o loʻo faʻapipiʻiina le tagata PDGF-BB (GenBank: CAA45383.1) na faʻaalia i se polyhistidine tag i le C-terminus.
Molecular Mass	O le Recombinant Human PDGF-BB e aofia ai le 115 amino acids ma valoia le molecular mass o le 13.1 kDa.
QC Su'ega Mama	> 90 % e pei ona fuafuaina e le SDS-PAGE.
Endotoxin
Gaoioiga	Fuafuaina e ala i le faʻamalosia o fualaʻau faʻalagolago i le faʻateleina o sela Balb / c 3T3. Ole ED₅₀ fa'amoemoeina mo lenei aafiaga ole ≤100ng/mL.
Fuafuaga	Lyophilized mai le sterile PBS, pH 7.4. E masani lava 6% mannitol e faʻaopoopoina e fai ma puipuiga aʻo leʻi faia le lyophilization.
Maumau	Lyophilized sauniuniga e mafai ona teuina i le -20 ℃. 6 masina i le -20 ℃ i lalo o tulaga fa'ama'i pe a uma ona toe fa'aleleia. 12 masina i le -80 ℃ i lalo o tulaga mama pe a uma ona toe faʻaleleia.
Teuga	Fautuaina e aliquot le polotini i ni aofaiga laiti pe a uma ona toe faʻafouina i le vai mo tui, saline masani poʻo le PBS, ma faʻatumauina le faʻamaʻi faʻafefe i luga ole 100μg / mL. Aloese mai ta'amilosaga fa'a'aisa-liu fa'afia.