Recombinant Human SCF Porotini

Fa'asoa: O lo'o iai Fa'asoa
Fa'ata'ita'iga: GMP-TL504

Faafesootai matou

Fa'amatalaga

Fa'amaumauga Fa'amaumauga

Fa'ailoga

Faila o Pepa

Stem Cell Factor, e taʻua foi o le SCF, kit-ligand, KL, steel factor, KITLG, FPH2, KL-1, Kitl, MGF, SCF, SF, poʻo le SHEP7, o se mole mole dimeric e faʻaaogaina ana galuega faʻaola e ala i le fusia ma faʻagaoioia le receptor tyrosine kinase c-Kit. O le faʻamalosia o le c-Kit e taʻitaʻia ai lona autophosphorylation ma le amataina o le faʻaliliuina o faʻailoga. Fa'ailoga polotini e fa'auluina e fa'agaoioia le c-Kit e nisi vaega o feso'ota'iga e fa'apipi'i fa'apitoa i toega tyrosine phosphorylated i totonu ole vaega o le c-Kit. O le fusifusia o le SCF i le C-kit e faʻaosofia ai le dimerization o le tali ma le autophosphorylation o toega tyrosine i le cytoplasmic domain. Tyrosine phosphorylation e amataina le tele o auala faʻailoga e aofia ai RAS, PI3 kinase, Src, ma JAK / STAT. Ae ui i lea, o le SCF o se mea faʻapitoa i le faʻavasegaina o le tele o ituaiga sela faʻapitoa e pei ole spermatogonial stem cell ma megakaryocyte progenitors. E ese mai le eseesega, e mafai foʻi e le SCF ona faʻatumauina le malosi i sela. Mo le fa'aoga ile falema'i, ole SCF e fa'aogaina fa'atasi ma isi cytokines e fa'atupu ai sela fa'agata ole myeloid mai le toto o le tagata. O lo'o fa'aogaina fo'i le SCF e fa'atupuina sela T mo togafitiga fa'avae sela, su'esu'ega o vaila'au ma fa'ata'ita'iga fa'ama'i. I suʻesuʻega faʻafouina, o loʻo faʻaaogaina le SCF i le hydrogel faʻamalolo manuʻa e avea o se auala e faʻateleina ai lona malosi pipii ma le toe faʻafouina o aano.

Cytokines
Fa'aaliga Host	HEK293 sela
upu uiga tutusa	C-kitligand,DCUA,FPH2,FPHH,Kitl,KL-1,MGF,SCF,SF,SHEP7
Polotini Fa'asologa	O se DNA faʻasologa o loʻo faʻapipiʻiina le SCF tagata (NP_000890.1) na faʻaalia i se Ana-tag i le C-terminus.
Molecular Mass	O le polotini SCF tagata toe tu'ufa'atasia e aofia ai le 170 amino acids ma valoia le mole mole mamafa o le 19.3 kD.
QC Su'ega Mama	> 90 % e pei ona fuafuaina e le SDS-PAGE.
Endotoxin
Gaoioiga	Fuaina i se su'esu'ega fa'ateleina o le sela e fa'aaoga ai sela Mo7e, e fetaui ma se gaioiga o le ≥2.0x10⁵IU/mg.
Fuafuaga	Lyophilized mai le sterile PBS, pH 7.4. E masani lava 6% mannitol e fa'aopoopoina e fai ma puipuiga a'o le'i faia le lyophilization.
Maumau	Lyophilized sauniuniga e mafai ona teuina i le -20 ℃. 6 masina i le -20 ℃ i lalo o tulaga fa'ama'i pe a uma ona toe fa'aleleia. 12 masina i le -80 ℃ i lalo o tulaga mama pe a uma ona toe faʻaleleia.
Teuga	Fautuaina e aliquot le polotini i ni aofaiga laiti pe a uma ona toe faʻafouina i le vai mo tui, saline masani poʻo le PBS, ma faʻatumauina le faʻamaʻi faʻafefe i luga ole 100μg / mL. Aloese mai ta'amilosaga fa'a'aisa-liu fa'afia.