Imibuzo Evame Ukubuzwa - T&L Biotechnology

Imibuzo yokucaphuna ◢

Q.
1. Luyini uhlobo oluncane lwamasosha omzimba e-CD3?

A.
CD3E
Q.
2. Ingabe kungcono ukumboza amasosha omzimba ancibilikayo e-CD3 nama-CD28 epuletini noma ukuwenza asebenze esixazululweni se-culture medium lapho esetshenziselwa ukuhlakulela ama-T cell omuntu?

A.
Zombili izindlela zingenzeka
Q.
3. Ibhodlela lamasiko lingagcinwa isikhathi esingakanani ngekhithi ye-NK reagent 2.0-A factor?

A.
Ngeke ibe khona inkinga lingakapheli isonto.
Q.
4. Ingabe ama-PBMC avuselelwe ngemva kwe-cryopreservation angasetshenziswa ukutshala ama-NK cell?

A.
Ama-PBMC agcinwe e-cryopreserved futhi avuselelwa angasetshenziselwa isiko lamaseli e-NK, kodwa umphumela wesiko awumuhle njengama-PBMC amasha.
Q.
5. Izengezo ze-MSC zingagcinwa isikhathi esingakanani ku-4 ℃ ngemva kokuvulwa?

A.
Izinsuku eziyisikhombisa.

Q.
6. Iyiphi i-buffer esetshenziselwa ukuchithwa kwe-recombinant human protein ye-Vitronectin (inombolo yomkhiqizo i-GMP-TL651)? Ingabe zikhona izidingo ze-calcium ne-magnesium ions?

A.
I-DPBS noma i-serum-free culture medium ingasetshenziswa. Akunconywa ukuqukatha i-calcium ne-magnesium ions ohlelweni lwe-buffer.
Q.
7. Indlela yokuguqula i-ED50 ibe yiyunithi?

A.
Iyunithi/mg=106/ED50(ng/ml)
Q.
8. Kuyini ukuhlanzeka kweseli nokusebenza kahle kokuhlukanisa ngemva kokuhlukaniswa kobuhlalu obukazibuthe?

A.
Ubumsulwa buchazwa njengengxenye yamaseli okuqondiwe aqoqwe futhi angahlaziywa nge-flow cytometry. Ukusebenza kahle kokuhlukanisa kuchazwa njengengxenye yamaseli aqondiwe atholiwe ngokuhlobene namaseli okuqondiwe kusampula yoqobo.
Q.
9. Buyini ubumsulwa bamaseli e-T ngemva kokusebenzisa ukuhlukaniswa kwe-GMP-TL603 & nokwenza kusebenze ubuhlalu obuzibuthe?

A.
Ukuhlanzeka kuyahlukahluka kuye ngesilinganiso seseli ukusebenza/isimo/T sesampula.
Q.
10. Kufanele senze nini ukuhlolwa kokudluliswa kwegciwane ngemva kokusebenzisa ukuhlukaniswa kwe-GMP-TL603 & nokwenza kusebenze ubuhlalu obuzibuthe ngesikhathi sokuhlolwa kwe-CAR-T? Ingabe sidinga ukususa ubuhlalu kazibuthe phakathi nenqubo yokudluliswa kwegciwane?

A.
Amakhasimende enza ucwaningo lokudluliswa kwegciwane ngo-24h, 48h noma 72h, kanti u-48h kuyiwona ovame kakhulu. Lapho igciwane lidluliselwa, ubuhlalu kazibuthe bungagcinwa.

Q.
11. Ingabe ubuhlalu obukazibuthe busasebenza uma bubekwe ku -20 ℃?

A.
Akunconyelwe ukusetshenziswa futhi.
Q.
12. Ingabe amaseli anelebuli ye-GMP-TL603 angatholwa ngokuqondile nge-flow cytometry?

A.
Ayikwazi, Kusikisela ukususa ubuhlalu kazibuthe kuqala ngaphambi kokuhlola amaseli
Q.
13. Ingabe ubuhlalu obukazibuthe be-GMP-TL603 budinga ukususwa lapho kuqoqwa amaseli ngemva kokwenziwa kusebenze nokunwetshwa?

A.
Ubuhlalu obuzibuthe budinga ukususwa, ubuhlalu obujwayelekile kazibuthe nohlaka lukazibuthe kungahlangabezana nohlelo lokusebenza.
Q.
14. Ungakanani usayizi wezinhlayiyana ze-NanoSep™ CD3/4/8 zokuhlukanisa ubuhlalu kazibuthe (TL-622, TL-623, TL-624)? Ingabe ubuhlalu obuzibuthe budinga amakholomu okuhlukanisa? Ingabe ubuhlalu obuzibuthe buyakwazi ukubola?

A.
50nm. Ubuhlalu obuzibuthe budinga ukudlula kumakholomu okuhlukanisa (Ihambisana namakholomu e-Miltenyi MACS), abolayo.
Q.
15. Ingabe ubuhlalu obuzibuthe buye benza ukuqinisekiswa kokuphepha?

A.
Ubuhlalu obunqunu bobuhlalu kazibuthe be-micrometer (GMP-TL603) kanye nobuhlalu obuzibuthe be-nano (TL-622, TL-623, TL-624) buqedele ukuhlola nokuqinisekisa ukuphepha ezingeni lezilwane.